Keypath p16/Ki-67 Immunoassay Test Kit(ICC) Detection principle Using immunological antigen and antibody specific combination principle, and through antibody recombination enzyme marker color system to form complex, add enzyme substrate (chromogenic agent) to make the color to treat the detection of antigen localization, qualitative. p16 protein is expressed in the nucleus and cytoplasm. HPV E7 protein can cause abnormal overexpression of p16